Translational Protein Research

Protein N-terminal acetylation From molecular mechanisms to human disease Most proteins are chemically modified in the cell and such modifications are often crucial for the protein’s ability to carry out a function. N-terminal acetylation one of the most common modifications in eukaryotes. It is catalyzed by N-terminal acetyltransferases (NATs) which are linked to cancer, genetic syndromes, and regulation of human metabolism.

The eukaryotic NAT-machinery — Photo: NAT-group

Affiliation

About the research group

Translational Protein Research group (Arnesen lab) is part of the research unit at the Department of Biomedicine.

Areas of research

Protein N-terminal acetylation

Protein N-terminal acetylation is among the most common types of protein modifications occurring on approximately 50 % of all yeast proteins and more than 80 % of all human proteins. Surprisingly, there is yet no clear functional understanding of how N-terminal acetylation affects proteins in general, although recent data indicate roles in protein folding, turnover and subcellular targeting. N-terminal acetylation is a co-translational process occurring on the ribosomes and is considered irreversible. However, we recently identified the first human enzymes acting post-translationally. An N-terminal acetyltransferase (NAT) transfers an Acetyl group from Acetyl Coenzyme A to the alpha-amino group of the N-terminal amino acid residue of the protein.
During the last two decades, all human NATs have been identified and characterized by our group. In humans as in yeast, three NAT complexes NatA, NatB and NatC are believed to perform most N-terminal acetylations. Each NAT complex is composed of specific subunits and acetylates a specific subset of substrates. A number of studies have described various aspects of N-terminal acetylation in humans, such as substrates, the molecular mechanisms, impact for protein fate, cellular roles, and connections to cancer and neurodegenerative disease. Through these studies, a complex and specific system of N-terminal acetylation has been revealed. The importance of N-terminal acetylation in human cell biology and disease is increasingly recognized.

Active research projects

The focus of our group is centered around protein N-terminal acetylation, both revealing the molecular and biological concepts of this protein modification as well as studying it in a human disease-perspective:
-    The molecular mechanisms of N-terminal acetylation
-    The functional role of N-terminal acetylation at the single protein level
-    Characterization of all human NATs (subunit composition, substrate specificity etc.)
-    The cellular impact of the human NATs (KD/KO phenotypes)
-    The role of NATs in cancer and other diseases
-    Development and use of NAT-specific inhibitors

A second project is centered around the molecular biology and clinical aspects of different endocrine tumours, in particular adrenal and thyroid tumours. This project is based on a patient biobank at the Department of Surgery, Haukeland University Hospital, and is a collaboration between HUS, MED (Biomed/K2) and MatNat (Bio).

We acknowledge research support from the European Research Council (ERC Consolidator Grant), the Research Council of Norway (Toppforsk), the Norwegian Cancer Society, Novo Nordisk Foundation and Helse Vest.

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Publications

Selected publications

Klein M, Wild K, McTiernan N, Arnesen T, Sinning I (2026) Nature Commun 17(1):884.

McTiernan N, Kjosås I, Arnesen T (2025) Nature Commun 16(1):703.

Øye H, Lundekvam M, Caiella A, Hellesvik M, Arnesen T (2025) Trends Biochem Sci 50(4):290-310.

Myllykoski M*, Lundekvam M, Osberg C, Nilsen SS, Arnesen T* (2025) Nature Commun 16(1):5960.

Heathcote KC, Keeley TP, Myllykoski M, Lundekvam M, McTiernan N, Akter S, Masson N, Ratcliffe PJ*, Arnesen T*, Flashman E* (2024) Nature Commun 15(1):5360.

Chelban V*, Aksnes H*, Maroofian R, LaMonica LC, Seabra L, Siggervåg A, Devic P, Shamseldin HE, Vandrovcova J, Murphy D, Richard AC, Quenez O, Bonnevalle A, Zanetti MN, Kaiyrzhanov R, Salpietro V, Efthymiou S, Schottlaender LV, Morsy H, Scardamaglia A, Tariq A, Pagnamenta AT, Pennavaria A, Krogstad LS, Bekkelund ÅK, Caiella A, Glomnes N, Brønstad KM, Tury S, Moreno De Luca A, Boland-Auge A, Olaso R, Deleuze JF, Anheim M, Cretin B, Vona B, Alajlan F, Abdulwahab F, Battini JL, İpek R, Bauer P, Zifarelli G, Gungor S, Kurul SH, Lochmuller H, Da'as SI, Fakhro KA, Gómez-Pascual A, Botía JA, Wood NW, Horvath R, Ernst AM, Rothman JE, McEntagart M, Crow YJ, Alkuraya FS, Nicolas G; SYNaPS Study Group; Arnesen T*, Houlden H* (2024) Nature Commun 15(1):2269. Commentary: Mov Disord, .

Kind L, Molnes J, Tjora E, Raasakka A, Myllykoski M, Colclough K, Saint-Martin C, Adelfalk C, Dusatkova P, Pruhova S, Valtonen-André C, Bellanné-Chantelot C, Arnesen T*, Kursula P, Njølstad PR* (2024) JCI Insight 9(11):e175278.

Arnesen T, Kjosås I, McTiernan N (2024) Nat Rev Mol Cell Biol 25(5):335-336.

Ree R*, Lin S-J, Dahl LOS, Huang K, Petree C, Varshney GK*, Arnesen T* (2024) Life Sci Alliance 7(12):e202402795.

Varland S*, Silva RD*, Kjosås I, Faustino A, Bogaert A, Billmann M, Boukhatmi H, Kellen B, Costanzo M, Drazic A, Osberg C, Chan K, Zhang X, Tong AHY, Andreazza S, Lee JJ, Nedyalkova L, Ušaj M, Whitworth AJ, Andrews BJ, Moffat J, Myers CL, Gevaert K, Boone C, Martinho RG*, Arnesen T* (2023) Nature Commun 14(1):6774. Editor's Highlights. Invited commentary: Nat Rev Mol Cell Biol,

Guzman UH, Aksnes H, Ree R, Krogh N, Jakobsson ME, Jensen LJ, Arnesen T*, Olsen JV* (2023) Nature Commun 14(1):4517. Editor's Highlights.

Aksnes H, McTiernan N, Arnesen T (2023) J Cell Sci 136 (14): jcs260766

Van Damme P, Osberg C, Jonckheere V, Glomnes N, Gevaert K, Arnesen T, Aksnes H (2023) . J Biol Chem 299(2):102824

Arnesen T, Aksnes H, Giglione C (2023) Trends Biochem Sci 48(6):495-499

Kind L, Raasakka A, Molnes J, Aukrust I, Bjørkhaug L, Njølstad PR, Kursula P, Arnesen T (2022) J Biol Chem 298(4):101803

McTiernan N, Tranebjærg L, Bjørheim AS, Hogue JS, Wilson WG, Schmidt B, Boerrigter MM, Nybo ML, Smeland MF, Tümer Z, Arnesen T (2022) Hum Genet 141(8):1355-1369.

Drazic A, Timmerman E, Kajan U, Marie M, Varland S, Impens F, Gevaert K, Arnesen T (2022) J Mol Biol 434(2):167397.

Morrison J, Altuwaijri NK, Brønstad K, Aksnes H, Alsaif HS, Evans A, Hashem M, Wheeler PG, Webb BD, Alkuraya FS, Arnesen T (2021) Genet Med 23(11):2213-2218.

Kweon HY, Lee MN, Dorfel M, Seo S, Gottlieb L, PaPazyan T, McTiernan N, Ree R, Bolton D, Garcia A, Flory M, Crain J, Sebold A, Lyons S, Ismail A, Marchi E, Sonn SK, Jeong SJ, Jeon S, Ju S, Conway SJ, Kim T, Kim HS, Lee C, Roh TY, Arnesen T, Marmorstein R, Oh GT, Lyon GJ (2021) eLife 10:e65952.

Ward TL, Tai W, Morton SU, Impens F, VanDamme P, Van Haver D, Timmerman E, Venturini G, Zhang K, Jang MY, Willcox JA, Haghighi A, Gelb BD, Chung WK, Goldmuntz E, Porter GA, Lifton R, Brueckner M, Yost HJ, Bruneau BG, Gorham J, Kim Y, Pereira AC, Homsy J, Benson CC, DePalma SR, Varland S, Chen CS, Arnesen T, Gevaert K, Seidman CE, Seidman JG (2021) Circ Res 128(8):1156-1169. Commentary by Timothy Cashman and Chinmay Trivedi (2021) Circ Res 128(8):1170–1172.&�Բ��;

McTiernan N, Gill H, Prada CE, Pachajoa H, Lores J, CAUSES study, Arnesen T (2021) Eur J Hum Genet 29(2):280-288.

Deng S, McTiernan N, Wei X, Arnesen T, Marmorstein R (2020) Nature Commun 11:818.

Rebowski G, Boczkowska M, Drazic A, Ree R, Goris M, Arnesen T, Dominguez R (2020) Science Adv 6:eaay8793.

Ree R, Kind L, Kaziales A, Varland S, Dai M, Richter K, Drazic A, Arnesen T (2020) J Biol Chem 295(49):16713-16731.

Beigl T, Hellesvik M, Saraste J, Arnesen T, Aksnes H (2020) Exp Cell Res 390(2):111961.

Krtenic B, Drazic A, Arnesen T, Reuter N (2020) PLoS Comp Biol 16(12):e1007988.

Aksnes H, Ree R, Arnesen T (2019) Mol Cell 73(6):1097-1114.

Drazic A, Aksnes H, Marie M, Boczkowska M, Varland S, Timmerman E, Foyn H, Glomnes N, Rebowski G, Impens F, Gevaert K, Dominguez R, Arnesen T (2018) Proc Natl Acad Sci USA 115(17):4399-4404. Front page and Commentaries: Proc Natl Acad Sci USA 115 (17) 4314-4316, and Cytoskeleton (Hoboken) doi: 10.1002/cm.21455.

Goris M, Magin RS, Foyn H, Myklebust LM, Varland S, Ree R, Drazic A, Bhambra P, Støve SI, Baumann M, Haug BE, Marmorstein R, Arnesen T (2018) Proc Natl Acad Sci USA 115(17):4405-4410.

Varland S, Aksnes H, Kryuchkov F, Impens F, Van Haver D, Jonckheere V, Ziegler M, Gevaert K, Van Damme P, Arnesen T (2018) Mol Cell Proteomics 17(12):2309-2323.

Ree R, Varland S, Arnesen T (2018) Exp Mol Med 50(7):90.

Cheng H, Dharmadhikari AV, Varland S, Ma N, Domingo D, Kleyner R, Rope AF, Yoon M, Stray-Pedersen A, Posey JE, Crews SR, Eldomery MK, Akdemir ZC, Lewis AM, Sutton VR, Rosenfeld JA, Conboy E, Agre K, Xia F, Walkiewicz M, Longoni M, High FA, van Slegtenhorst MA, Mancini GMS, Finnila CR, van Haeringen A, den Hollander N, Ruivenkamp C, Naidu S, Mahida S, Palmer EE, Murray L, Lim D, Jayakar P, Parker MJ, Giusto S, Stracuzzi E, Romano C, Beighley JS, Bernier RA, Küry S, Nizon M, Corbett MA, Shaw M, Gardner A, Barnett C, Armstrong R, Kassahn KS, Van Dijck A, Vandeweyer G, Kleefstra T, Schieving J, Jongmans MJ, de Vries BBA, Pfundt R, Kerr B, Rojas SK, Boycott KM, Person R, Willaert R, Eichler EE, Kooy F, Yang Y, Wu JC, Lupski JR, Arnesen T, Cooper GM, Chung WK, Gecz J, Stessman HAF, Meng L, Lyon GJ (2018) Am J Hum Genet DOI:

Aksnes H, Goris M, Strømland Ø, Drazic A, Waheed Q, Reuter N, Arnesen T (2017) J Biol Chem 292(16):6821-6837.

Aksnes H, Drazic A, Marie M, Arnesen T (2016) Trends Biochem Sci 41(9):746-60.

Støve SI, Magin R, Foyn H, Haug BE, Marmorstein R, Arnesen T (2016) Structure 24(7):1044-56.

Van Damme P, Kalvik TV, Starheim KK, Jonckheere V, Myklebust LM, Menschaert G, Varhaug JE, Gevaert K, Arnesen T (2016) Mol Cell Proteomics 15(11):3361-3372.

Saunier C, Støve SI, Popp B, Gérard B, Blenski M, AhMew N, de Bie C, Goldenberg P, Isidor B, Keren B, Leheup B, Lampert L, Mignot C, Tezcan K, Mancini GM, Nava C, Wasserstein M, Bruel AL, Thevenon J, Masurel A, Duffourd Y, Kuentz P, Huet F, Rivière JB, van Slegtenhorst M, Faivre L, Piton A, Reis A, Arnesen T, Thauvin-Robinet C, Zweier C (2016) Hum Mutat 37(8):755-64.

Drazic A, Myklebust LM, Ree R, Arnesen T (2016) Biochim Biophys Acta 1864(10):1372-401.

Aksnes H, Van Damme P, Goris M, Starheim KK, Marie M, Støve SI, Hoel C, Kalvik TV, Hole K, Glomnes N, Furnes C, Ljostveit S, Ziegler M, Niere M, Gevaert K, Arnesen T (2015) Cell Rep 10(8):1362-74.

Linster E, Stephan I, Bienvenut WV, Maple-Grødem J, Myklebust LM, Huber M, Reichelt M, Sticht C, Geir Møller S, Meinnel T, Arnesen T, Giglione C, Hell R, Wirtz M (2015) Nature Commun 6:7640.

Myklebust LM, Van Damme P, Støve SI, Dörfel MJ, Abboud A, Kalvik TV, Grauffel C, Jonckheere V, Wu Y, Swensen J, Kaasa H, Liszczak G, Marmorstein R, Reuter N, Lyon GJ, Gevaert K, Arnesen T (2015) Hum Mol Genet 24(7):1956-76.

Van Damme P, Hole K, Gevaert K, Arnesen T (2015) Proteomics 15(14):2436-46.

Varland S, Osberg C, Arnesen T (2015) Proteomics 15(14):2385-401.

Van Damme P, Støve SI, Glomnes N, Gevaert K, Arnesen T (2014) Mol Cell Proteomics 13(8):2031-41.

Liszczak G, Goldberg JM, Foyn H, Petersson EJ, Arnesen T, Marmorstein R (2013) Nature Struct Mol Biol 20(9):1098-105.

Foyn H, Jones JE, Lewallen D, Narawane R, Varhaug JE, Thompson PR, Arnesen T (2013) ACS Chem Biol 8:1121-1127.

Kalvik TV and Arnesen T (2013) Oncogene 32:269–276.

Foyn H, Van Damme P, Støve S, Glomnes N, Evjenth R, Gevaert K, Arnesen T (2013) Mol Cell Proteomics 12:42-54. Covered by

Starheim KK, Gevaert K, Arnesen T (2012) Trends Biochem Sci 37(4):152-61. Selected by the Editorial Board to the top ten TiBS articles of 2012.

Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui-Artola A, Kim DS, De Juan-Pardo E, Demeyer K, Hole K, Larrea E, Timmerman E, Prieto J, Arnesen T, Sherman F, Gevaert K, Aldabe R (2012) Proc Natl Acad Sci USA 109(31):12449-54.

Arnesen T (2011) PLoS Biology 9(5):e1001074.

Rope A, Wang K, Evjenth R, Xing J, Johnston JJ, Swensen JJ, Johnson WE, Moore B, Huff CD, Bird LM, Carey JC, Opitz JM, Stevens CA, Jiang T, Schank C, Fain HD, Robison R, Dalley B, Chin S, South ST, Pysher TJ, Jorde LB, Hakonarson H, Lillehaug JR, Biesecker LB, Yandell M, Arnesen T, Lyon GJ (2011) Am J Hum Genet 89(1):28-43. Comment in Nature News:

Van Damme P, Hole K, Pimenta-Marques A, Helsens K, Vandekerckhove J, Martinho RG, Gevaert K, Arnesen T (2011) PLoS Genet 7(7):e1002169.

Liszczak G, Arnesen T, Marmorstein R (2011) J Biol Chem 286(42):37002-10.

Van Damme P, Evjenth R, Foyn H, Demeyer K, De Bock P-J, Lillehaug JR, Vandekerckhove J, Arnesen T, Gevaert K (2011) Mol Cell Proteomics 10:M110.004580.

Arnesen T, Starheim KK, Van Damme P, Evjenth R, Dinh H, Betts M, Ryningen A, Vandekerckhove J, Gevaert K, Anderson D (2010) Mol Cell Biol 30(8):1898-909.

Arnesen T, Van Damme P, Polevoda B, Helsens K, Evjenth R, Colaert N, Varhaug JE, Vandekerckhove J, Lillehaug JR, Sherman F, Gevaert K (2009) Proc Natl Acad Sci USA 106:8157-8162.

Starheim K, Gromyko D, Ryningen A, Varhaug JE, Lillehaug JR, Arnesen T (2009) Mol Cell Biol 29(13):3569-81.

Evjenth R, Hole K, Karlsen OA, Ziegler M, Arnesen T, Lillehaug JR (2009) J Biol Chem. 284(45):31122-9.

Arnesen T, Gromyko D, Pendino F, Ryningen A, Varhaug JE, Lillehaug JR. (2006) Oncogene 25:4350-4360.

Arnesen T, Anderson D, Baldersheim C, Lanotte M, Varhaug JE, Lillehaug JR. (2005) Biochemical J 386(Pt 3):433-43.

All publications

Peer reviewed publications (chronological order):

1. Arnesen T, Anderson D, Baldersheim C, Lanotte M, Varhaug JE, Lillehaug JR. (2005) Identification and characterization of the human ARD1-NATH protein acetyltransferase complex. Biochemical J 386(Pt 3):433-43.

2. Furnes C, Arnesen T, Askjaer P, Kjems J, Szilvay AM. (2005) HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain. Retrovirology 2(1):39.

3. Arnesen T, Gromyko D, Horvli O, Fluge Ø, Lillehaug JR, Varhaug JE (2005) Expression of NATH and hARD1 proteins in thyroid neoplasms. Thyroid 15(10):1131-6.

4. Arnesen T, Kong X, Evjenth R, Gromyko D, Varhaug JE, Lin Z, Sang N, Caro J, Lillehaug JR. (2005) Interaction between HIF-1α (ODD) and hARD1 does not induce acetylation and destabilization of HIF-1α. FEBS Lett 579(28):6428-32.

5. Arnesen T, Anderson D, Torsvik J, Varhaug JE, Lillehaug JR. (2006) Cloning and characterization of hNAT5/hSAN: An evolutionary conserved component of the NatA protein N-α-acetyltransferase complex. Gene 371:291-295.

6. Arnesen T, Gromyko D, Pendino F, Ryningen A, Varhaug JE, Lillehaug JR. (2006) Induction of apoptosis in human cells by RNAi mediated knock-down of hARD1 and NATH, components of the protein N-α-acetyltransferase complex. Oncogene 25:4350-4360.

7. Arnesen T. (2006) HIF-1α and ARD1: enemies, friends or neither? Nat Rev Cancer. 6 (3): doi:10.1038/nrc1779-c1.

8. Arnesen T, Betts M, Pendino F, Liberles D, Anderson D, Caro J, Kong X, Varhaug JE, Lillehaug JR. (2006) Characterization of hARD2, a processed hARD1 gene duplicate encoding a human protein N-α-acetyltransferase. BMC Biochem 7:13.

9. Knappskog S, Geisler J, Arnesen T, Lillehaug JR, Lønning PE. (2006) A novel type of deletion in the CDKN2A gene identified in a melanoma-prone family. Genes Chr. Cancer.45(12):1155-63.

10. Starheim K, Arnesen T, Gromyko D, Ryningen A, Varhaug JE, Lillehaug JR. (2008) Identification of the human N^a-acetyltransferase complex B (hNatB) – a complex important for cell cycle progression. Biochemical J 415:325-331.

11. Arnesen T, Thompson PR, Varhaug JE, Lillehaug JR. (2008) The protein acetyltransferase ARD1 – a novel cancer drug target? Curr Cancer Drug Targets. 8:545-553.

12. Målen H, Lillehaug JR, Arnesen T. (2009)The protein N^α-terminal acetyltransferase hNaa10p (hArd1) is phosphorylated in HEK293 cells. BMC Res Notes2:32.

13. Arnesen T, Van Damme P, Polevoda B, Helsens K, Evjenth R, Colaert N, Varhaug JE, Vandekerckhove J, Lillehaug JR, Sherman F, Gevaert K. (2009) Proc Natl Acad Sci USA. 106:8157-8162.

14. Starheim K, Gromyko D, Ryningen A, Varhaug JE, Lillehaug JR, Arnesen T. (2009) Knockdown of the human NatC protein N^α-terminal acetyltransferase complex induces p53-mediated apoptosis and aberrant hArl8b localization. Mol Cell Biol 29(13):3569-81.

15. Arnesen T, Gromyko D, Kagabo D, Betts MJ, Varhaug JE, Anderson D, Lillehaug JR. (2009) A novel human NatA N^α-terminal acetyltransferase complex: hNaa16p-hNaa10p (hNat2-hArd1). BMC Biochem. 10:15.

16. Takubo K, Tsuchiya H, Kurimasa A, Arnesen T, Ryoke K, Shiota G. (2009) Involvement of N-acetyltransferase human in the cytotoxic activity of 5-fluorouracil. Anti-Cancer Drugs. 20:668-75.

17. Arnesen T. (2009) Protein N-terminal acetylation: NAT 2007–2008 Symposia. BMC Proc. 3 (Suppl 6):S1.

18. Polevoda B, Arnesen T, Sherman F. (2009) A synopsis of eukaryotic Nα-terminal acetyltransferases: nomenclature, subunits and substrates. BMC Proc. 3 (Suppl 6):S2.

19. Starheim KK, Gromyko D, Velde R, Varhaug JE, Arnesen T. (2009) Composition and biological significance of the human N^α-terminal acetyltransferases. BMC Proc. 3 (Suppl 6):S3

20. Evjenth R, Hole K, Karlsen OA, Ziegler M, Arnesen T, Lillehaug JR (2009) Human Naa50 (Nat5/San) displays both protein N-alpha and N-epsilon acetyltransferase activity. J Biol Chem. 284(45):31122-9.

21. Arnesen T, Starheim KK, Van Damme P, Evjenth R, Dinh H, Betts M, Ryningen A, Vandekerckhove J, Gevaert K, Anderson D. (2010) The chaperone-like protein HYPK acts together with NatA in cotranslational N-terminal acetylation and prevention of Huntingtin aggregation. Mol Cell Biol 30(8):1898-909.

22. Gromyko D, Arnesen T, Ryningen A, Varhaug JE, Lillehaug JR (2010) Depletion of the human N-terminal acetyltransferase A (hNatA) induces p53-dependent apoptosis and p53-independent growth inhibition. Int J Cancer 127(12):2777-89.

23. Van Damme P, Evjenth R, Foyn H, Demeyer K, De Bock P-J, Lillehaug JR, Vandekerckhove J, Arnesen T, Gevaert K. (2011) Proteome-derived peptide libraries allow detailed analysis of the substrate specificities of Nα-acetyltransferases and point to hNaa10p as the posttranslational actin Nα-acetyltransferase. Mol Cell Proteomics 10: M110.004580.

24. Helsens K, Van Damme P, Degroeve S, Martens L, Arnesen T, Vandekerckhove J, Gevaert K. (2011) Bioinformatics analysis of a Saccharomyces cerevisiae N-terminal proteome provides evidence of alternative translation initiation and post-translational N-terminal acetylation. J Proteome Res 10(8):3578-3589.

25. Arnesen T. (2011) PLoS Biology 9(5): e1001074.

26. Rope A, Wang K, Evjenth R, Xing J, Johnston JJ, Swensen JJ, Johnson WE, Moore B, Huff CD, Bird LM, Carey JC, Opitz JM, Stevens CA, Jiang T, Schank C, Fain HD, Robison R, Dalley B, Chin S, South ST, Pysher TJ, Jorde LB, Hakonarson H, Lillehaug JR, Biesecker LB, Yandell M, Arnesen T, Lyon GJ (2011) Am J Hum Genet 89(1):28-43. Comment in Nature News:

27. Van Damme P, Arnesen T, Gevaert K (2011) FEBS J 278(20):3822-34.

28. Van Damme P, Hole K, Pimenta-Marques A, Helsens K, Vandekerckhove J, Martinho RG, Gevaert K, Arnesen T (2011) PLoS Genet 7(7): e1002169.

29. Hole K, Van Damme P, Dalva M, Aksnes H, Glomnes N, Varhaug J, Lillehaug JR, Gevaert K, Arnesen T (2011) PLoS ONE 6(9): e24713.

30. Liszczak G, Arnesen T, Marmorstein R (2011) J Biol Chem 286(42):37002-10.

31. Evjenth R, Brenner A, Thompson PR, Arnesen T, Frøystein NÅ, Lillehaug JR (2012) J Biol Chem 287(13):10081-8.

32. Starheim KK, Gevaert K, Arnesen T (2012) Trends Biochem Sci 37(4):152-61.

33. Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui-Artola A, Kim DS, De Juan-Pardo E, Demeyer K, Hole K, Larrea E, Timmerman E, Prieto J, Arnesen T, Sherman F, Gevaert K, Aldabe R (2012) Proc Natl Acad Sci USA 109(31):12449-54.

34. Grauffel C, Abboud A, Liszczak G, Marmorstein R, Arnesen T, Reuter N (2012) PLoS ONE 7(12): e52642

35. Kalvik TV and Arnesen T (2013) Oncogene 32(3):269-76.

36. Foyn H, Van Damme P, Støve S, Glomnes N, Evjenth R, Gevaert K, Arnesen T (2013) Mol Cell Proteomics 12:42-54. Covered by .

37. Van Damme P, Arnesen T, Ruttens B, Gevaert K (2013) Methods Mol Biol 981:115-26.

38. Evjenth R, Van Damme P, Gevaert K, Arnesen T (2013) Methods Mol Biol 981:95-102.

39. Foyn H, Jones JE, Lewallen D, Narawane R, Varhaug JE, Thompson PR, Arnesen T (2013) ACS Chem Biol 8:1121–1127.

40. Aksnes H, Osberg C, Arnesen T (2013) PLOS ONE 8(4): e61012.

41. Arnesen T, Glomnes N, Strømsøy S, Knappskog S, Heie A, Akslen LA, Grytaas M, Varhaug JE, Gimm O, Brauckhoff M (2013) Langenbecks Arch Surg 398(6):869-74.

42. Liszczak G, Goldberg JM, Foyn H, Petersson EJ, Arnesen T, Marmorstein R (2013) Nature Struct Mol Biol 20(9):1098-105.

43. Dutta RK, Welander J, Brauckhoff M, Arnesen T, Alesina P, Walz M, Söderkvist P, Gimm O (2014) Endocr Relat Cancer ERC 21(1):L1-4.

44. Van Damme P, Støve SI, Glomnes N, Gevaert K, Arnesen T (2014) Mol Cell Proteomics 13(8):2031-41.

45. Popp B, Støve SI, Endele S, Myklebust LM, Hoyer J, Sticht H, Azzarello-Burri S, Rauch A, Arnesen T, Reis A (2015) Eur J Hum Genet 23(5):602-9.

46. Aksnes H, Hole K, Arnesen T (2015) Int Rev Cell Mol Biol 316:267-305.

47. Myklebust LM, Van Damme P, Støve SI, Dörfel MJ, Abboud A, Kalvik TV, Grauffel C, Jonckheere V, Wu Y, Swensen J, Kaasa H, Liszczak G, Marmorstein R, Reuter N, Lyon GJ, Gevaert K, Arnesen T (2015) Hum Mol Genet 24(7):1956-76.

48. Aksnes H, Van Damme P, Goris M, Starheim KK, Marie M, Støve SI, Hoel C, Kalvik TV, Hole K, Glomnes N, Furnes C, Ljostveit S, Ziegler M, Niere M, Gevaert K, Arnesen T (2015) Cell Rep 10(8):1362-74.

49. Van Damme P, Hole K, Gevaert K, Arnesen T (2015) Proteomics 15(14):2436-46.

50. Varland S, Osberg C, Arnesen T (2015) Proteomics 15(14):2385-401.

51. Aksnes H, Drazic A, Arnesen T (2015) Trends Biochem Sci 40(8):422-4.

52. Aksnes H, Marie M, Arnesen T (2015) Oncotarget 6(18):15726-7.

53. Linster E, Stephan I, Bienvenut WV, Maple-Grødem J, Myklebust LM, Huber M, Reichelt M, Sticht C, Geir Møller S, Meinnel T, Arnesen T, Giglione C, Hell R, Wirtz M (2015) Nature Commun 6:7640.

54. Ree RM, Myklebust LM, Thiel P, Foyn H, Fladmark KE, Arnesen T (2015) Biosci Rep 35(5). pii: e00249.

55. Myklebust LM, Støve SI, Arnesen T (2015) Oncotarget 6(33):34041-2.

56. Casey JP, Støve SI, McGorrian C, Galvin J, Blenski M, Dunne A, Ennis S, Brett F, King MD, Arnesen T, Lynch SA (2015) Sci Rep 5:16022.

57. Saunier C, Støve SI, Popp B, Gérard B, Blenski M, AhMew N, de Bie C, Goldenberg P, Isidor B, Keren B, Leheup B, Lampert L, Mignot C, Tezcan K, Mancini GM, Nava C, Wasserstein M, Bruel AL, Thevenon J, Masurel A, Duffourd Y, Kuentz P, Huet F, Rivière JB, van Slegtenhorst M, Faivre L, Piton A, Reis A, Arnesen T, Thauvin-Robinet C, Zweier C (2016) Hum Mutat 37(8):755-64.

58. Drazic A, Myklebust LM, Ree R, Arnesen T (2016) Biochim Biophys Acta 1864(10):1372-401.

59. Støve SI, Magin R, Foyn H, Haug BE, Marmorstein R, Arnesen T (2016) Structure 24(7):1044-56.

60. Aksnes H, Drazic A, Marie M, Arnesen T (2016) Trends Biochem Sci 41(9):746-60.

61. Osberg C, Aksnes H, Ninzima S, Marie M, Arnesen T (2016) Sci Rep 6:31627.

62. Van Damme P, Kalvik TV, Starheim KK, Jonckheere V, Myklebust LM, Menschaert G, Varhaug JE, Gevaert K, Arnesen T (2016) Mol Cell Proteomics 15(11):3361-3372.

63. Ribeiro AL, Silva RD, Foyn H, Tiago MN, Rathore OS, Arnesen T, Martinho RG (2016) Sci Rep 6:39118.

64. Drazic A, Arnesen T (2017) [¹⁴C]-acetyl-coenzymeA-based in vitro N-terminal acetylation assay Methods Mol Biol 1574:1-8.

65. Foyn H, Thompson PR, Arnesen T (2017) DTNB-Based quantification of in vitro enzymatic N-terminal acetyltransferase activity Methods Mol Biol 1574:9-15.

66. Aksnes H, Goris M, Strømland Ø, Drazic A, Waheed Q, Reuter N, Arnesen T (2017) J Biol Chem 292(16):6821-6837.

67. Starheim KK, Kalvik T, Bjørkøy G, Arnesen T (2017) Biosci Rep 37(2). pii: BSR20170066.

68. Grytaas MA, Strømsøy SS, Rørvik JT, Arnes JB, Heie A, Arnesen T, Jørstad MD, Nedrebø BG, Jøssang DE, Jensen DK, Rørvik HD, Sagen JV, Mellgren G, Thordarson HB, Husebye ES, Løvås K (2017) Horm Metab Res 49(11):838-846.

69. Varland S, Myklebust LM, Strømsøy S, Glomnes N, Torsvik J, Varhaug JE, Arnesen T (2018) Gene 644:27-37.

70. McTiernan N, Støve SI, Aukrust I, Mårli MT, Myklebust LM, Houge G, Arnesen T (2018) BMC Med Genet 19(1):47.

71. Drazic A, Aksnes H, Marie M, Boczkowska M, Varland S, Timmerman E, Foyn H, Glomnes N, Rebowski G, Impens F, Gevaert K, Dominguez R, Arnesen T (2018) Proc Natl Acad Sci USA 115(17):4399-4404. Issue front page and Commentary by Peter A. Rubenstein and Kuo-kuang Wen (2018) Proc Natl Acad Sci USA 115 (17) 4314-4316.

72. Goris M, Magin RS, Foyn H, Myklebust LM, Varland S, Ree R, Drazic A, Bhambra P, Støve SI, Baumann M, Haug BE, Marmorstein R, Arnesen T (2018) Proc Natl Acad Sci USA 115(17):4405-4410. Commentary by Peter A. Rubenstein and Kuo-kuang Wen (2018) Proc Natl Acad Sci USA 115 (17) 4314-4316.

73. Cheng H, Dharmadhikari AV, Varland S, Ma N, Domingo D, Kleyner R, Rope AF, Yoon M, Stray-Pedersen A, Posey JE, Crews SR, Eldomery MK, Akdemir ZC, Lewis AM, Sutton VR, Rosenfeld JA, Conboy E, Agre K, Xia F, Walkiewicz M, Longoni M, High FA, van Slegtenhorst MA, Mancini GMS, Finnila CR, van Haeringen A, den Hollander N, Ruivenkamp C, Naidu S, Mahida S, Palmer EE, Murray L, Lim D, Jayakar P, Parker MJ, Giusto S, Stracuzzi E, Romano C, Beighley JS, Bernier RA, Küry S, Nizon M, Corbett MA, Shaw M, Gardner A, Barnett C, Armstrong R, Kassahn KS, Van Dijck A, Vandeweyer G, Kleefstra T, Schieving J, Jongmans MJ, de Vries BBA, Pfundt R, Kerr B, Rojas SK, Boycott KM, Person R, Willaert R, Eichler EE, Kooy F, Yang Y, Wu JC, Lupski JR, Arnesen T, Cooper GM, Chung WK, Gecz J, Stessman HAF, Meng L, Lyon GJ (2018) Am J Hum Genet 102(5):985-994.

74. Støve SI, Blenski M, Stray-Pedersen A, Wierenga KJ, Jhangiani SN, Akdemir ZC, Crawford D, McTiernan N, Myklebust LM, Purcarin G, McNall-Knapp R, Wadley A, Belmont JW, Kim JJ, Lupski JR, Arnesen T (2018) Eur J Hum Genet 26(9):1294-1305.

75. Varland S, Arnesen T (2018) BMC Res Notes 11:404.

76. Ree R, Varland S, Arnesen T (2018) Exp Mol Med 50(7):90.

77. Arnesen T, Marmorstein R, Dominguez R (2018) Cytoskeleton 75(7):318-322.

78. Varland S, Aksnes H, Kryuchkov F, Impens F, Van Haver D, Jonckheere V, Ziegler M, Gevaert K, Van Damme P, Arnesen T (2018) Mol Cell Proteomics 17(12):2309-2323.

79. Aksnes H, Marie M, Arnesen T, Drazic A (2018) Commun Integr Biol 11(4):e1526572.

80. Ochaya S, Franzén O, Buhwa DA, Foyn H, Butler CE, Støve SI, Tyler KM, Arnesen T, Matovu E, Åslund L, Andersson B (2019) J Parasitol Res 2019:6594212.

81. Aksnes H, Ree R, Arnesen T (2019) Mol Cell 73(6):1097-1114.

82. Ree R, Geithus AS, Tørring PM, Sørensen KP, Damkjær M, DDD study, Lynch SA, Arnesen T (2019) BMC Med Genet 20(1):101.

83. Dutta RK, Arnesen T, Heie A, Walz MK, Alesina P, Söderkvist P, Gimm O (2019) Eur J Endocrinol 181:K37–K41. Commentary by Ute Scholl (2019) Eur J Endocrinol 181(5):C21-C22.

84. Deng S, McTiernan N, Wei X, Arnesen T, Marmorstein R (2020) Nature Commun 11:818.

85. Abboud A, Bedoucha P, Byska J, Arnesen T, Reuter N (2020) Comput Struct Biotechnol J 18:532-547.

86. Beigl T, Hellesvik M, Saraste J, Arnesen T, Aksnes H (2020) Exp Cell Res 390(2):111961.

87. Rebowski G, Boczkowska M, Drazic A, Ree R, Goris M, Arnesen T, Dominguez R (2020) Science Adv 6:eaay8793.

88. Bader I, McTiernan N, Darbakk C, Boltshauser E, Ree R, Ebner S, Mayr JA, Arnesen T (2020) BMC Med Genet 21(1):153.

89. Ree R, Kind L, Kaziales A, Varland S, Dai M, Richter K, Drazic A, Arnesen T (2020) J Biol Chem 295(49):16713-16731.

90. McTiernan N, Darbakk C, Ree R, Arnesen T (2020) Int J Mol Sci 21(23), 8973

91. Krtenic B, Drazic A, Arnesen T, Reuter N (2020) PLoS Comp Biol 16(12):e1007988.

92. McTiernan N, Gill H, Prada CE, Pachajoa H, Lores J, CAUSES study, Arnesen T (2021) Eur J Hum Genet 29(2):280-288.

93. Velasco K, St-Louis JL, Hovland HN, Thompson N, Ottesen Å, Choi MH, Pedersen L, Njølstad PR, Arnesen T, Fjeld K, Aukrust I, Myklebust LM, Molven A (2021) J Inherit Metab Dis 44(1):240-252.

94. Ward TL, Tai W, Morton SU, Impens F, VanDamme P, Van Haver D, Timmerman E, Venturini G, Zhang K, Jang MY, Willcox JA, Haghighi A, Gelb BD, Chung WK, Goldmuntz E, Porter GA, Lifton R, Brueckner M, Yost HJ, Bruneau BG, Gorham J, Kim Y, Pereira AC, Homsy J, Benson CC, DePalma SR, Varland S, Chen CS, Arnesen T, Gevaert K, Seidman CE, Seidman JG (2021) Circ Res 128(8):1156-1169. Commentary by Timothy Cashman and Chinmay Trivedi (2021) Circ Res 128(8):1170–1172.&�Բ��;

95. Dutta RK, Larsson M, Arnesen T, Heie A, Walz M, Alesina P, Gimm O, Söderkvist P (2021) Sci Rep 11(1):10562.

96. Morrison J, Altuwaijri NK, Brønstad K, Aksnes H, Alsaif HS, Evans A, Hashem M, Wheeler PG, Webb BD, Alkuraya FS, Arnesen T (2021) Genet Med 23(11):2213-2218.

97. Kweon HY, Lee MN, Dorfel M, Seo S, Gottlieb L, PaPazyan T, McTiernan N, Ree R, Bolton D, Garcia A, Flory M, Crain J, Sebold A, Lyons S, Ismail A, Marchi E, Sonn SK, Jeong SJ, Jeon S, Ju S, Conway SJ, Kim T, Kim HS, Lee C, Roh TY, Arnesen T, Marmorstein R, Oh GT, Lyon GJ (2021) eLife 10:e65952.

98. Ree R, Krogstad K, McTiernan N, Jakobsson ME, Arnesen T (2021) Int J Mol Sci 22(21):11805.

99. Drazic A, Timmerman E, Kajan U, Marie M, Varland S, Impens F, Gevaert K, Arnesen T (2022) J Mol Biol 434(2):167397.

100. McTiernan N, Tranebjærg L, Bjørheim AS, Hogue JS, Wilson WG, Schmidt B, Boerrigter MM, Nybo ML, Smeland MF, Tümer Z, Arnesen T (2022) Hum Genet 141(8):1355-1369.

101. Kind L, Raasakka A, Molnes J, Aukrust I, Bjørkhaug L, Njølstad PR, Kursula P, Arnesen T (2022) J Biol Chem 298(4):101803

102. Van Damme P, Osberg C, Jonckheere V, Glomnes N, Gevaert K, Arnesen T, Aksnes H (2023) . J Biol Chem 299(2):102824

103. Arnesen T, Aksnes H (2023) Trends Biochem Sci 48(5):414-416

104. Arnesen T, Aksnes H, Giglione C (2023) Trends Biochem Sci 48(6):495-499

105. D'Onofrio G, Cuccurullo C, Larsen SK, Severino M, D'Amico A, Brønstad K, AlOwain M, Morrison JL, Wheeler PG, Webb BD, Alfalah A, Iacomino M, Uva P, Coppola A, Merla G, Salpietro VD, Zara F, Striano P, Accogli A, Arnesen T, Bilo L (2023) Clin Genet 104(3):371-376

106. Lundekvam M, Arnesen T, McTiernan N (2023) Methods Enzymol 686:29-43

107. Varland S, Brønstad KM, Skinner SJ, Arnesen T (2023) Am J Med Genet A 191(9):2402-2410.

108. Myklebust LM, Baumann M, Støve SI, Foyn H, Arnesen T, Haug BE (2023) Front Chem 11:1202501

109. Aksnes H, McTiernan N, Arnesen T (2023) J Cell Sci 136 (14): jcs260766

110. Guzman UH, Aksnes H, Ree R, Krogh N, Jakobsson ME, Jensen LJ, Arnesen T*, Olsen JV* (2023) Nature Commun 14(1):4517. Editor's Highlights.

111. Kind L, Driver M, Raasakka A, Onck PR, Njølstad PR, Arnesen T, Kursula P (2023) Front Mol Biosci 10:1249939.

112. Varland S, Silva RD, Kjosås I, Faustino A, Bogaert A, Billmann M, Boukhatmi H, Kellen B, Costanzo M, Drazic A, Osberg C, Chan K, Zhang X, Tong AHY, Andreazza S, Lee JJ, Nedyalkova L, Ušaj M, Whitworth AJ, Andrews BJ, Moffat J, Myers CL, Gevaert K, Boone C, Martinho RG*, Arnesen T* (2023) Nature Commun 14(1):6774. Editor's Highlights. Invited commentary: Nat Rev Mol Cell Biol,

113. Chelban V*, Aksnes H*, Maroofian R, LaMonica LC, Seabra L, Siggervåg A, Devic P, Shamseldin HE, Vandrovcova J, Murphy D, Richard AC, Quenez O, Bonnevalle A, Zanetti MN, Kaiyrzhanov R, Salpietro V, Efthymiou S, Schottlaender LV, Morsy H, Scardamaglia A, Tariq A, Pagnamenta AT, Pennavaria A, Krogstad LS, Bekkelund ÅK, Caiella A, Glomnes N, Brønstad KM, Tury S, Moreno De Luca A, Boland-Auge A, Olaso R, Deleuze JF, Anheim M, Cretin B, Vona B, Alajlan F, Abdulwahab F, Battini JL, İpek R, Bauer P, Zifarelli G, Gungor S, Kurul SH, Lochmuller H, Da'as SI, Fakhro KA, Gómez-Pascual A, Botía JA, Wood NW, Horvath R, Ernst AM, Rothman JE, McEntagart M, Crow YJ, Alkuraya FS, Nicolas G; SYNaPS Study Group; Arnesen T*, Houlden H* (2024) Nature Commun 15(1):2269. Commentary: Mov Disord, .

114. Arnesen T, Kjosås I, McTiernan N (2024) Nat Rev Mol Cell Biol 25(5):335-336.

115. Lyon GJ, Longo J, Garcia A, Inusa F, Marchi E, Shi D, Dörfel M, Arnesen T, Aldabe R, Lyons S, Nashat MA, Bolton D (2024) PLOS ONE 19(5):e0301328.

116. Kind L, Molnes J, Tjora E, Raasakka A, Myllykoski M, Colclough K, Saint-Martin C, Adelfalk C, Dusatkova P, Pruhova S, Valtonen-André C, Bellanné-Chantelot C, Arnesen T*, Kursula P, Njølstad PR* (2024) JCI Insight 9(11):e175278.

117. Heathcote KC, Keeley TP, Myllykoski M, Lundekvam M, McTiernan N, Akter S, Masson N, Ratcliffe PJ*, Arnesen T*, Flashman E* (2024) Nature Commun 15(1):5360.

118. Larsen SK, Bekkelund ÅK, Glomnes N, Arnesen T, Aksnes H (2024) Biochimie 226:113-120.

119. Ree R, Lin S-J, Dahl LOS, Huang K, Petree C, Varshney GK, Arnesen T (2024) Life Sci Alliance 7(12):e202402795.

120. McTiernan N, Kjosås I, Arnesen T (2025) Nature Commun 16(1):703.

121. Øye H, Lundekvam M, Caiella A, Hellesvik M, Arnesen T (2025) Trends Biochem Sci 50(4):290-310.

122. Myllykoski M, Lundekvam M, Osberg C, Nilsen SS, Arnesen T (2025) Nature Commun 16(1):5960.

123. Klein M, Wild K, McTiernan N, Arnesen T, Sinning I (2026) Nature Commun 17(1):884.